Instrument: Illumina HiSeq 2500
Strategy: RNA-Seq
Source: TRANSCRIPTOMIC
Selection: cDNA
Layout: PAIRED
Construction protocol: Hyphae were flash frozen and RNA was harvested using Nucleospin RNA Plant kit (Macherey-Nagel, Germany). Roots were flash frozen and RNA extracted using TRIzol reagent. Illumina TruSeq RNA Sample Prep Kit was used with 1 - 3ug of total RNA for the construction of sequencing libraries. RNA libraries were prepared for sequencing using standard Illumina protocols; Illumina TruSeq RNA Sample Prep Kit was used with the total RNA upto 2µg from the “confrontation in soil” and upto 3µg from the “tripartite interaction in soil” experiments in order to construct the sequencing libraries.